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fluorescent protein gfp epitope  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd fluorescent protein gfp epitope
    Fluorescent Protein Gfp Epitope, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+protein+gfp+epitope/Protein+A+(recombinant)/pm20881252-99-33-42
    Average 94 stars, based on 237 article reviews
    fluorescent protein gfp epitope - by Bioz Stars, 2026-10
    94/100 stars

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    Transduction:

    Article Title: Role of UBC9 in the regulation of the adipogenic program in 3T3-L1 adipocytes.
    Article Snippet: .. Cell transduction with recombinant adenoviruses The recombinant adenoviruses were generated by cloning the NotI/HindIII fragments encoding either wild-type mouse UBC9 or the C93A UBC9 mutant into the transfer vector pAdTrackCMV containing the green fluorescent protein (GFP) epitope using the AdEasy vector system (Q-Biogene, Montreal, Quebec, Canada), according to the manufacturer’s instructions. .. The negative mutant form of mouse UBC9, UBC9Ala93, containing Ala instead of Cys93, was generated by PCR based site-directed mutagenesis by using the QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), as previously described (15).

    Recombinant:

    Article Title: Role of UBC9 in the regulation of the adipogenic program in 3T3-L1 adipocytes.
    Article Snippet: .. Cell transduction with recombinant adenoviruses The recombinant adenoviruses were generated by cloning the NotI/HindIII fragments encoding either wild-type mouse UBC9 or the C93A UBC9 mutant into the transfer vector pAdTrackCMV containing the green fluorescent protein (GFP) epitope using the AdEasy vector system (Q-Biogene, Montreal, Quebec, Canada), according to the manufacturer’s instructions. .. The negative mutant form of mouse UBC9, UBC9Ala93, containing Ala instead of Cys93, was generated by PCR based site-directed mutagenesis by using the QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), as previously described (15).

    Generated:

    Article Title: Role of UBC9 in the regulation of the adipogenic program in 3T3-L1 adipocytes.
    Article Snippet: .. Cell transduction with recombinant adenoviruses The recombinant adenoviruses were generated by cloning the NotI/HindIII fragments encoding either wild-type mouse UBC9 or the C93A UBC9 mutant into the transfer vector pAdTrackCMV containing the green fluorescent protein (GFP) epitope using the AdEasy vector system (Q-Biogene, Montreal, Quebec, Canada), according to the manufacturer’s instructions. .. The negative mutant form of mouse UBC9, UBC9Ala93, containing Ala instead of Cys93, was generated by PCR based site-directed mutagenesis by using the QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), as previously described (15).

    Cloning:

    Article Title: Role of UBC9 in the regulation of the adipogenic program in 3T3-L1 adipocytes.
    Article Snippet: .. Cell transduction with recombinant adenoviruses The recombinant adenoviruses were generated by cloning the NotI/HindIII fragments encoding either wild-type mouse UBC9 or the C93A UBC9 mutant into the transfer vector pAdTrackCMV containing the green fluorescent protein (GFP) epitope using the AdEasy vector system (Q-Biogene, Montreal, Quebec, Canada), according to the manufacturer’s instructions. .. The negative mutant form of mouse UBC9, UBC9Ala93, containing Ala instead of Cys93, was generated by PCR based site-directed mutagenesis by using the QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), as previously described (15).

    Mutagenesis:

    Article Title: Role of UBC9 in the regulation of the adipogenic program in 3T3-L1 adipocytes.
    Article Snippet: .. Cell transduction with recombinant adenoviruses The recombinant adenoviruses were generated by cloning the NotI/HindIII fragments encoding either wild-type mouse UBC9 or the C93A UBC9 mutant into the transfer vector pAdTrackCMV containing the green fluorescent protein (GFP) epitope using the AdEasy vector system (Q-Biogene, Montreal, Quebec, Canada), according to the manufacturer’s instructions. .. The negative mutant form of mouse UBC9, UBC9Ala93, containing Ala instead of Cys93, was generated by PCR based site-directed mutagenesis by using the QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), as previously described (15).

    Plasmid Preparation:

    Article Title: Role of UBC9 in the regulation of the adipogenic program in 3T3-L1 adipocytes.
    Article Snippet: .. Cell transduction with recombinant adenoviruses The recombinant adenoviruses were generated by cloning the NotI/HindIII fragments encoding either wild-type mouse UBC9 or the C93A UBC9 mutant into the transfer vector pAdTrackCMV containing the green fluorescent protein (GFP) epitope using the AdEasy vector system (Q-Biogene, Montreal, Quebec, Canada), according to the manufacturer’s instructions. .. The negative mutant form of mouse UBC9, UBC9Ala93, containing Ala instead of Cys93, was generated by PCR based site-directed mutagenesis by using the QuickChange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), as previously described (15).



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